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Cdc34 Polyclonal Antibody

Cdc34 Polyclonal Antibody

Views(15) Publications(0) Catalog no(ABP52920)
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Specification

Product name Cdc34 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Cdc34
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CDC34; UBCH3; UBE2R1; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ubiquitin-conjugating enzyme E2 R1 encoded by CDC34 is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Gene ID 997
Alternative CDC34; UBCH3; UBE2R1; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
Others Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein.
Accession P49427
Observed Band(KD) 34

Image & description

Fig.1. Western Blot analysis of K562,  PC-3, Mouse liver cells using Cdc34 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of K562, PC-3, Mouse liver cells using Cdc34 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.

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