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CD34 Polyclonal Antibody

CD34 Polyclonal Antibody

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Specification

Product name CD34 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human CD34
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 36 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CD34 molecule encoded by CD34 may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for CD34.
Gene ID 947
Alternative CD34; Hematopoietic progenitor cell antigen CD34; CD antigen CD34
Others CD34 Polyclonal Antibody detects endogenous levels of CD34 protein.
Accession P28906

Image & description

Fig.1. Western Blot analysis of various cells using CD34 Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using CD34 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of HepG2 cells using CD34 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of HepG2 cells using CD34 Polyclonal Antibody diluted at 1:2000.

Fig.3. mouse-lung mouse-brain mouse-spleen mouse-kidney.

Fig.3. mouse-lung mouse-brain mouse-spleen mouse-kidney.

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