| Product name | CD31 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human CD31 around the non-phosphorylation site of Y713 |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | ELISA, IF, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Platelet and endothelial cell adhesion molecule 1 encoded by PECAM1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. |
| Gene ID | 5175 |
| Alternative | PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31 |
| Others | CD31 Polyclonal Antibody detects endogenous levels of CD31 protein. |
| Accession | P16284 |
| Observed Band(KD) | 85-130 |
Fig.1. Western Blot analysis of various cells using CD31 Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of rat-musle cells using CD31 Polyclonal Antibody diluted at 1:1000.
Fig.3. Western blot analysis of KB lysis using CD31 antibody. Antibody was diluted at 1:1000.
Fig.4. Western blot analysis of 293T lysis using CD31 antibody. Antibody was diluted at 1:1000.
Author：YQ Tan, F Wang, RJ Ma, J Zhang, G Zhou Publication name：Int Immunopharmacol IF：3.943
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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