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CD1D Polyclonal Antibody

CD1D Polyclonal Antibody

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Specification

Product name CD1D Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human CD1D
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CD1D; Antigen-presenting glycoprotein CD1d; R3G1; CD1d

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CD1D encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID 912
Alternative CD1D; Antigen-presenting glycoprotein CD1d; R3G1; CD1d
Others CD1D Polyclonal Antibody detects endogenous levels of CD1D protein.
Accession P15813
Observed Band(KD) 37

Image & description

Fig.1. Western Blot analysis of K562 cells using CD1D Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of K562 cells using CD1D Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western blot analysis of SH-SY5Y lysis using CD1D antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western blot analysis of SH-SY5Y lysis using CD1D antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

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