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CD133 Polyclonal Antibody

CD133 Polyclonal Antibody

Views(334) Publications(0) Catalog no(ABP52923)
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Specification

Product name CD133 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human CD133
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PROM1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PROM1 encodes a pentaspan transmembrane glycoprotein. Prominin-1 localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID 8842
Alternative PROM1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133
Others CD133 Polyclonal Antibody detects endogenous levels of CD133 protein.
Accession O43490
Observed Band(KD) 100

Image & description

Fig. Western Blot analysis of A549, SW620, HT29, Jurkat, Mouse kidney, Mouse lung cells using CD133 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western Blot analysis of A549, SW620, HT29, Jurkat, Mouse kidney, Mouse lung cells using CD133 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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