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Cathepsin L Polyclonal Antibody

Cathepsin L Polyclonal Antibody

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Specification

Product name Cathepsin L Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Cathepsin L
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CTSL1; CTSL; Cathepsin L1; Major excreted protein; MEP

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cathepsin L1 encoded by CTSL1 is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene.
Gene ID 1514
Alternative CTSL1; CTSL; Cathepsin L1; Major excreted protein; MEP
Others Cathepsin L Polyclonal Antibody detects endogenous levels of Cathepsin L protein.
Accession P07711
Observed Band(KD) 37

Image & description

Fig.1. Western Blot analysis of H460, K562 cells using Cathepsin L Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of H460, K562 cells using Cathepsin L Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

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