| Product name | CaMKIIα/β/δ (phospho Thr305) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human CaMKIIα/β/δ around the phosphorylation site of T305 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; Ca |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 54kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Calcium/calmodulin dependent protein kinase II beta belongs to the serine/threonine protein kinase family and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of CAMK2B is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. |
| Gene ID | 816/817 |
| Alternative | CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; Ca |
| Others | Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody detects endogenous levels of CaMKIIα/β/δ protein only when phosphorylated at T305. |
| Accession | Q9UQM7/Q13554/Q13557 |
| Observed Band(KD) | 54 |
Fig.1. Western Blot analysis of various cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of 453 cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000.
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