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C/EBP β (phospho Thr235) Polyclonal Antibody

C/EBP β (phospho Thr235) Polyclonal Antibody

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Specification

Product name C/EBP β (phospho Thr235) Polyclonal Antibody
Immunogen Synthesized peptide derived from human C/EBP β around the phosphorylation site of T235
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CEBPB; LAP; TCF5; PP9092; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background This intronless gene (CEBPB) encodes a transcription factor (CCAAT/enhancer binding protein beta) that contains a basic leucine zipper (bZIP) domain. CCAAT/enhancer binding protein beta functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.
Gene ID 1051
Alternative CEBPB; LAP; TCF5; PP9092; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5
Others Phospho-C/EBP β (T235) Polyclonal Antibody detects endogenous levels of C/EBP β protein only when phosphorylated at T235.
Accession P17676
Observed Band(KD) 36

Image & description

Fig. Western Blot analysis of various cells using Phospho-C/EBP β (T235) Polyclonal Antibody diluted at 1:500.

Fig. Western Blot analysis of various cells using Phospho-C/EBP β (T235) Polyclonal Antibody diluted at 1:500.

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