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C/EBP α Polyclonal Antibody

C/EBP α Polyclonal Antibody

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Product name C/EBP α Polyclonal Antibody
Immunogen Synthesized peptide derived from human C/EBP α around the non-phosphorylation site of S21
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CEBPA encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The CCAAT/enhancer binding protein alpha functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.
Gene ID 1050
Alternative CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
Others C/EBP α Polyclonal Antibody detects endogenous levels of C/EBP α protein.
Accession P49715
Observed Band(KD) 42+30

Image & description

Fig. Western Blot analysis of 293 cells using C/EBP α Polyclonal Antibody.

Fig. Western Blot analysis of 293 cells using C/EBP α Polyclonal Antibody.

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