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Bub3 Polyclonal Antibody

Bub3 Polyclonal Antibody

Views(43) Publications(0) Catalog no(ABP50801)
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Specification

Product name Bub3 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Bub3 at AA range: 260-340
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative BUB3; Mitotic checkpoint protein BUB3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background BUB3 encodes a protein involved in spindle checkpoint function. The encoded protein (BUB3, mitotic checkpoint protein) contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene ID 9184
Alternative BUB3; Mitotic checkpoint protein BUB3
Others Bub3 Polyclonal Antibody detects endogenous levels of Bub3 protein.
Accession O43684
Observed Band(KD) 40

Image & description

Fig.1. Western Blot analysis of various cells using Bub3 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Bub3 Polyclonal Antibody.

Fig.2. Western Blot analysis of 293 cells using Bub3 Polyclonal Antibody.

Fig.2. Western Blot analysis of 293 cells using Bub3 Polyclonal Antibody.

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