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BM28 Polyclonal Antibody

BM28 Polyclonal Antibody

Views(63) Publications(0) Catalog no(ABP50775)
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Specification

Product name BM28 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human BM28 at AA range: 1-80
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein (minichromosome maintenance complex component 2) encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Gene ID 4171
Alternative MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Others BM28 Polyclonal Antibody detects endogenous levels of BM28 protein.
Accession P49736
Observed Band(KD) 120

Image & description

Fig.1. Western Blot analysis of various cells using BM28 Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using BM28 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of 293 cells using BM28 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of 293 cells using BM28 Polyclonal Antibody diluted at 1:2000.

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