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BLM (phospho Thr99) Polyclonal Antibody

BLM (phospho Thr99) Polyclonal Antibody

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Product name BLM (phospho Thr99) Polyclonal Antibody
Immunogen Synthesized peptide derived from human BLM around the phosphorylation site of T99
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination.
Gene ID 641
Alternative BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3
Others Phospho-BLM (T99) Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at T99.
Accession P54132
Observed Band(KD) 159

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