|Product name||Beclin-1 Mouse Monoclonal Antibody (5C2)|
|Immunogen||Synthetic Peptide of Beclin-1 at AA range of 110-190|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen|
|Molecular weight||60 KD|
|Storage buffer||PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||BECN1 (beclin 1) encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.|
|Others||Beclin-1 protein detects endogenous levels of BECN1.|
Fig.1. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Beclin-1 Mouse mAb diluted at 1:200.
Fig.3. Western blot analysis of 1) 293T Cell Lysate, 2) C2C12 Cell Lysate, 3) Rat Brain Tissue Lysate using Beclin-1 Mouse mAb diluted at 1:2000.
Author：Ozkan S, Isildar B, Ercin M, et al. Publication name：Stem cell research & therapy IF：8.098
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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