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BCAS2 Polyclonal Antibody

BCAS2 Polyclonal Antibody

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Specification

Product name BCAS2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human BCAS2 at AA range: 150-230
Host Rabbit
Reactivity Human, Monkey, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative BCAS2; DAM1; Pre-mRNA-splicing factor SPF27; Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53- or –mutant cells; this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.
Gene ID 10286
Alternative BCAS2; DAM1; Pre-mRNA-splicing factor SPF27; Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27
Others BCAS2 Polyclonal Antibody detects endogenous levels of BCAS2 protein.
Accession O75934

Image & description

Fig.1. Western Blot analysis of various cells using BCAS2 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using BCAS2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of COS7 cells using BCAS2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of COS7 cells using BCAS2 Polyclonal Antibody diluted at 1:1000.

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