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ATP5F1 Polyclonal Antibody

ATP5F1 Polyclonal Antibody

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Specification

Product name ATP5F1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human ATP5F1 at AA range: 130-210
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ATP5F1; ATP synthase subunit b; mitochondrial; ATPase subunit b

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ATP5F1 (ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1) encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5F1 encodes the b subunit of the proton channel.
Gene ID 515
Alternative ATP5F1; ATP synthase subunit b; mitochondrial; ATPase subunit b
Others ATP5F1 Polyclonal Antibody detects endogenous levels of ATP5F1 protein.
Accession P24539

Image & description

Fig. Western Blot analysis of 3T3 cells using ATP5F1 Polyclonal Antibody diluted at 1:500.

Fig. Western Blot analysis of 3T3 cells using ATP5F1 Polyclonal Antibody diluted at 1:500.

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