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ATP5A Polyclonal Antibody

ATP5A Polyclonal Antibody

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Specification

Product name ATP5A Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human ATP5A at AA range: 180-260
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ATP5A1; ATP5A; ATP5AL2; ATPM; ATP synthase subunit alpha; mitochondrial

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ATP5A1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5A1 encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
Gene ID 498
Alternative ATP5A1; ATP5A; ATP5AL2; ATPM; ATP synthase subunit alpha; mitochondrial
Others ATP5A Polyclonal Antibody detects endogenous levels of ATP5A protein.
Accession P25705

Image & description

Fig.1. Western Blot analysis of various cells using ATP5A Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using ATP5A Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of RAW264.7 cells using ATP5A Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of RAW264.7 cells using ATP5A Polyclonal Antibody diluted at 1:500.

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