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ASIC1 Polyclonal Antibody

ASIC1 Polyclonal Antibody

Views(2) Publications(0) Catalog no(ABP57824)
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Specification

Product name ASIC1 Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human ASIC1 protein at amino acid sequence of 220-280
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,IHC-f,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:500-200;ELISA 1:10000-20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Acid-sensing ion channel 1; ASIC1; Amiloride-sensitive cation channel 2, neuronal; Brain sodium channel 2; BNaC2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 70-75kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ASIC1 (Acid Sensing Ion Channel Subunit 1) is a Protein Coding gene. Diseases associated with ASIC1 include Brain Injury and Frozen Shoulder. Among its related pathways are Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds and Ion channel transport. ASIC1 encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described.
Gene ID 41
Alternative Acid-sensing ion channel 1; ASIC1; Amiloride-sensitive cation channel 2, neuronal; Brain sodium channel 2; BNaC2
Others The antibody detects endogenous levels of ASIC1
Accession P78348

Image & description

Fig.1. Western blot analysis of SW480 lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.1. Western blot analysis of SW480 lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human-brain, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human-brain, antibody was diluted at 1:100.

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