| Product name | APC Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human APC at AA range: 2770-2850 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | APC; DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 310kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | APC encodes a tumor suppressor protein (APC, WNT signaling pathway regulator) that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in APC cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. |
| Gene ID | 324 |
| Alternative | APC; DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5 |
| Others | APC Polyclonal Antibody detects endogenous levels of APC protein. |
| Accession | P25054 |
Fig.1. Western Blot analysis of various cells using APC Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of COLO205 cells using APC Polyclonal Antibody diluted at 1:500.
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