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Apaf-1 Polyclonal Antibody

Apaf-1 Polyclonal Antibody

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Specification

Product name Apaf-1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Apaf-1
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background APAF1 encodes a cytoplasmic protein that initiates apoptosis. Apoptotic protease-activating factor 1 contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Gene ID 317
Alternative APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1
Others Apaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.
Accession O14727

Image & description

Fig.1. Western Blot analysis of K562 cells using Apaf-1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of K562 cells using Apaf-1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

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