Login Register
English
0

Cart

$ 0

Apaf-1-ALT Polyclonal Antibody

Apaf-1-ALT Polyclonal Antibody

Views(315) Publications(0) Catalog no(ABP50673)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Apaf-1-ALT Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Apaf-1-ALT at AA range: 260-340
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19; dJ179L10.2; likely ortholog of mouse monocyte macrophage 19

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background APAF1 encodes a cytoplasmic protein that initiates apoptosis. This protein (apoptotic peptidase activating factor 1) contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Gene ID 317
Alternative APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19; dJ179L10.2; likely ortholog of mouse monocyte macrophage 19
Others Apaf-1-ALT Polyclonal Antibody detects endogenous levels of Apaf-1-ALT protein.
Accession O14727-6

Image & description

Fig.1. Western Blot analysis of various cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of COLO205 cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of COLO205 cells using Apaf-1-ALT Polyclonal Antibody diluted at 1:500.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Apaf-1-ALT Polyclonal Antibody”