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Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

Views(1987) Publications(31) Catalog no(ABL1050)
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Specification

Product name Anti-Plant Actin Mouse Monoclonal Antibody (3T3)
Immunogen Recombinant Protein
Host Mouse
Reactivity Arabidopsis, Brassica napus, Corn
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative AT3G12110; actin-11

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Gene ID 820385
Alternative AT3G12110; actin-11
Accession P53496
Observed Band(KD) 43

Image & description

Fig. Western blot analysis of plant Actin expression in Arabidopsis (lane 1-4) with Anti-Plant Actin Mouse Monoclonal Antibody (3T3) (A01050, 1:2000) and HRP, Goat Anti-Mouse IgG (A21010, 1:10000).

Fig. Western blot analysis of plant Actin expression in Arabidopsis (lane 1-4) with Anti-Plant Actin Mouse Monoclonal Antibody (3T3) (A01050, 1:2000) and HRP, Goat Anti-Mouse IgG (A21010, 1:10000).

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Tips for Abbkine antibodies

1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.

2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.

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