Product name | Anti-CBP Tag Monoclonal Antibody (12H5) |
Immunogen | Recombinant Protein |
Host | Mouse |
Applications | WB |
Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000). |
Clonality | Monoclonal |
Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
Alternative | CBP; CREB binding protein; Crebbp; Cyclic AMP responsive enhancer binding protein; KAT3A; RSTS; RTS; Rubinstein Taybi syndrome |
Formulation | Liquid solution |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins. |
Alternative | CBP; CREB binding protein; Crebbp; Cyclic AMP responsive enhancer binding protein; KAT3A; RSTS; RTS; Rubinstein Taybi syndrome |
Fig. Western blot analysis of CBP recombinant protein with anti-CBP tag monoclonal Antibody (12H5) at 1:5000 (lane A) and 1:10000 (lane B) dilutions, seperately.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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