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ANKRD30A Polyclonal Antibody

ANKRD30A Polyclonal Antibody

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Specification

Product name ANKRD30A Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human ANKRD30A at AA range: 1010-1090
Host Rabbit
Reactivity Human
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative ANKRD30A; Ankyrin repeat domain-containing protein 30A; Serologically defined breast cancer antigen NY-BR-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 153kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases, such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKRD30A (ankyrin repeat domain 30A), also known as serologically defined breast cancer antigen NY-BR-1, is a 1,397 amino acid coiled-coil protein. Containing six ANK repeats and thirty-seven exons, ANKRD30A is a distant homolog of the POTE family. Initiated by either Dnmt1 or Met-57, ANKRD30A is highly expressed in breast and testis, and faintly in placenta. Present in 80% of breast cancers, but not in other types of tumors, ANKRD30A is a differentiation antigen and a potential target for antibody‐based therapies in breast cancers.
Gene ID 91074
Alternative ANKRD30A; Ankyrin repeat domain-containing protein 30A; Serologically defined breast cancer antigen NY-BR-1
Others ANKRD30A Polyclonal Antibody detects endogenous levels of ANKRD30A protein.
Accession Q9BXX3

Image & description

Fig. Western Blot analysis of various cells using ANKRD30A Polyclonal Antibody diluted at 1:2000.

Fig. Western Blot analysis of various cells using ANKRD30A Polyclonal Antibody diluted at 1:2000.

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