| Product name | Aldolase A Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Aldolase A at AA range: 1-80 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ALDOA; ALDA; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 39kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by ALDOA, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. |
| Gene ID | 226 |
| Alternative | ALDOA; ALDA; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase |
| Others | Aldolase A Polyclonal Antibody detects endogenous levels of Aldolase A protein. |
| Accession | P04075 |
Fig.1. Western Blot analysis of various cells using Aldolase A Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of HT29 cells using Aldolase A Polyclonal Antibody diluted at 1:1000.
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