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Akt1 (phospho Tyr474) Polyclonal Antibody

Akt1 (phospho Tyr474) Polyclonal Antibody

Views(37) Publications(0) Catalog no(ABP0054)
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Specification

Product name Akt1 (phospho Tyr474) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Akt1 around the phosphorylation site of Y474
Host Rabbit
Reactivity Human, Monkey, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 60 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1.
Gene ID 207
Alternative AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Others Phospho-Akt1 (Y474) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at Y474.
Accession P31749

Image & description

Fig.1. Western Blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293T cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293T cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Fig.3. Western Blot analysis of KB using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

Fig.3. Western Blot analysis of KB using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1:1000.

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