| Product name | Actinin-α3 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Actinin-α3 at AA range: 10-90 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 103kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ACTN3 encodes a member of the alpha-actin binding protein gene family. Actinin alpha 3 (gene/pseudogene) is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of ACTN3 is associated with elite athlete status. |
| Gene ID | 89 |
| Alternative | ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein |
| Others | Actinin-α3 Polyclonal Antibody detects endogenous levels of Actinin-α3 protein. |
| Accession | Q08043 |
Fig.1. Western Blot analysis of various cells using Actinin-α3 Polyclonal Antibody.
Fig.2. Western Blot analysis of HT29 cells using Actinin-α3 Polyclonal Antibody.
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