| Product name | Actin Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Actin at AA range: 300-380 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Fish |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 45kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ACTB encodes one of six different actin proteins (actin beta). Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| Gene ID | 60/71 |
| Alternative | ACTB; Actin; cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin |
| Others | Actin Polyclonal Antibody detects endogenous levels of Actin protein. |
| Accession | P60709/Q9BYX7/P63261 |
Fig.1. Western Blot analysis of various cells using Actin Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1:2000.
Fig.3. Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000.
Fig.4. Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000.
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