| Product name | Actin β Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Actin β |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Chicken,Globefish,Bovine,Hamster,Pig,Ovine,Cat,Pig,Dog,Sheep |
| Applications | IF,WB,IHC,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200;WB 1:2000-1:10000;IHC 1:100-1:300;ELISA 1:20000;Not yet tested in other applications; |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ACTB; Actin; cytoplasmic 1; Beta-actin |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 42kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ACTB encodes one of six different actin proteins (actin beta). Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| Gene ID | 60 |
| Alternative | ACTB; Actin; cytoplasmic 1; Beta-actin |
| Others | Actin β Polyclonal Antibody detects endogenous levels of Actin β protein. |
| Accession | P60709 |
Fig.1. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Actin β Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.2. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, Actin β Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, Actin β Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Western Blot analysis of 1, hela 2, A549 3, HEPG2 4, mouse brain 5, mouse lung 6, mouse liver 7, rat brain 8, rat lung 9, rat liver cells using primary antibody diluted at 1:1000 (4°C overnight).
Author：BK Mishra, BD Banerjee, V Agrawal Publication name：Endocrine IF：2.55
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