| Product name | ACAT-1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human ACAT-1 at AA range: 190-270 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ACAT1; ACAT; MAT; Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; T2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 45kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ACAT1 encodes a mitochondrially localized enzyme (acetyl-CoA acetyltransferase 1) that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in ACAT1 are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. |
| Gene ID | 38 |
| Alternative | ACAT1; ACAT; MAT; Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; T2 |
| Others | ACAT-1 Polyclonal Antibody detects endogenous levels of ACAT-1 protein. |
| Accession | P24752 |
Fig.1. Western Blot analysis of various cells using ACAT-1 Polyclonal Antibody.
Fig.2. Western Blot analysis of A549 cells using ACAT-1 Polyclonal Antibody.
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