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14-3-3-pan (Acetyl Lys51/49) Polyclonal Antibody

14-3-3-pan (Acetyl Lys51/49) Polyclonal Antibody

Views(3) Publications(0) Catalog no(ABP57438)
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Specification

Product name 14-3-3-pan (Acetyl Lys51/49) Polyclonal Antibody
Immunogen Synthesized peptide derived from human 14-3-3-pan around the acetylation site of K51
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 30kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background YWHAB (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta) encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for YWHAB.
Gene ID 7529
Others Acetyl-14-3-3-pan (K51/49) Polyclonal Antibody detects endogenous levels of 14-3-3-pan around the acetylation site of K51 protein.
Accession P31946

Image & description

Fig. Western blot analysis of Mouse-SPLEEN using Acetyl-14-3-3-pan (K51/49) antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western blot analysis of Mouse-SPLEEN using Acetyl-14-3-3-pan (K51/49) antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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