| Product name | Human Tissue inhibitors of metalloproteinase 4 (TIMP4) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Tissue inhibitors of metalloproteinase 4 (TIMP4) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP4 in samples. An antibody specific for TIMP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | TIMP4; Tissue inhibitor of metalloproteinase 4 |
| Kit components | • Human Tissue inhibitors of metalloproteinase 4 microplate • Human Tissue inhibitors of metalloproteinase 4 standard • Human Tissue inhibitors of metalloproteinase 4 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Tissue inhibitors of metalloproteinase 4 (TIMP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIMP4. No significant cross-reactivity or interference between Human TIMP4 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Leco et al. (1997) cloned cDNAs encoding mouse Timp4. The predicted Timp4 protein contains the hallmarks of TIMP proteins, including 12 conserved cysteine residues and 2 short conserved sequence motifs. Northern blot analysis of adult mouse tissues detected Timp4 expression in brain, heart, ovary, and skeletal muscle. Olson et al., 1998 determined that the TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. They demonstrated a high degree of conservation of gene structure in the TIMP family. By FISH, Olson et al., 1998 mapped the TIMP4 gene to chromosome 3p25. By interspecific backcross analysis, they mapped the mouse Timp4 gene to chromosome 6 in a region of syntenic homology with human chromosome 3. |
| Gene ID | 7079 |
| Alternative | TIMP4; Tissue inhibitor of metalloproteinase 4 |
| Accession | Q99727 |
Fig.1. Human Tissue inhibitors of metalloproteinase 4 (TIMP4) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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