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Human Menin (MEN1) ELISA Kit

Human Menin (MEN1) ELISA Kit

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Specification

Product name Human Menin (MEN1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Menin (MEN1) ELISA Kit employs a two-site sandwich ELISA to quantitate MEN1 in samples. An antibody specific for MEN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MEN1; MEAI; SCG2; OTTHUMP00000070176; OTTHUMP00000070184; endocrine adenomatosis; multiple; menin

Product Properties

Kit components • Human Menin microplate
• Human Menin standard
• Human Menin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Menin (MEN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MEN1. No significant cross-reactivity or interference between Human MEN1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MEN1 encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of Menin is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants.
Gene ID 4221
Alternative MEN1; MEAI; SCG2; OTTHUMP00000070176; OTTHUMP00000070184; endocrine adenomatosis; multiple; menin
Accession O00255

Image & description

Fig.1. Human Menin (MEN1) Standard Curve.

Fig.1. Human Menin (MEN1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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