| Product name | CD25 Monoclonal Antibody |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Reactivity | Human |
| Applications | IHC,IF |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:200;IF 1:50-200 |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Alternative | IL2RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in IL2RA are associated with interleukin 2 receptor alpha deficiency. |
| Gene ID | 3559 |
| Alternative | IL2RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25 |
| Others | The antibody detects endogenous human CD25 protein. |
| Accession | P01589 |
Fig.1. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, CD25 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Spleentissue using CD 25 Mouse mAb diluted at 1:500.
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