Unleash Precision in VEGF Quantitation: Introducing EliKine™ Human VEGF ELISA Kit by Abbkine

For researchers delving into angiogenesis, cancer biology, or inflammatory disease studies, accurate quantification of Vascular Endothelial Growth Factor (VEGF) is non-negotiable. Enter the EliKine™ Human VEGF ELISA Kit (Catalog No.: KTE6033) from Abbkine—a game-changing tool designed to redefine reliability and sensitivity in VEGF detection. Built on the brand’s legacy of delivering high-performance research reagents, this kit empowers scientists to unlock critical insights into VEGF-mediated pathways with unparalleled confidence.

Crafted with a two-site sandwich ELISA design, this kit leverages the specificity of dual antibodies to target human VEGF, ensuring minimal cross-reactivity with analogs and irrelevant proteins. The assay workflow is streamlined yet robust: a VEGF-specific capture antibody is pre-coated onto microplate wells, capturing VEGF from samples such as serum, plasma, cell culture supernatants, or other biological fluids. Following incubation, a biotin-conjugated detection antibody binds to the captured VEGF, forming a stable ternary complex. Streptavidin-Horseradish Peroxidase (HRP) conjugate then binds to the biotinylated antibody, and the addition of a chromogenic substrate triggers a color reaction proportional to the amount of VEGF present. The intensity of the color is measured spectrophotometrically, enabling precise quantitative analysis—all within 3-5 hours, depending on experimental experience.

What sets the EliKine™ Human VEGF ELISA Kit apart is its exceptional sensitivity and specificity, hallmarks of Abbkine’s reagent development expertise. Rigorous validation ensures no significant interference from biological matrices or homologous proteins, guaranteeing trustworthy results even with low-abundance samples. The kit comes fully equipped with all essential components: pre-coated microplate, VEGF standard, detection antibody, Streptavidin-HRP, assay buffers, substrate solution, stop solution, wash buffer, and plate covers—eliminating the hassle of sourcing individual reagents and ensuring consistency across experiments.

Designed with researchers’ needs in mind, the kit balances performance with user-friendliness. Clear usage guidelines emphasize critical steps: allowing reagents to equilibrate to room temperature for 30 minutes, using fresh pipette tips to avoid contamination, and assaying samples and standards in duplicate or triplicate for statistical rigor. Storage is straightforward too—unopened kits remain stable at 2-8°C, while opened components can be preserved according to the detailed protocol, extending the kit’s usability. Shipped with gel packs and blue ice, the kit arrives in optimal condition, ready to deliver consistent results from the first use.

Beyond its technical prowess, the EliKine™ Human VEGF ELISA Kit aligns with Abbkine’s commitment to advancing research accessibility. Whether you’re investigating VEGF’s role in tumor angiogenesis, wound healing, or ischemic diseases, this kit provides the precision needed to drive impactful discoveries. Backed by the brand’s reputation for quality control and customer support, it’s not just a reagent—it’s a partner in pushing the boundaries of scientific inquiry.

In a field where data accuracy can make or break research outcomes, the EliKine™ Human VEGF ELISA Kit (KTE6033) stands out as an indispensable tool. It’s time to move beyond guesswork and embrace a quantitation solution that combines sensitivity, specificity, and ease of use. Elevate your VEGF research with Abbkine’s EliKine™ kit—where innovation meets reliability, one experiment at a time. Explore the full potential of your samples and accelerate your journey toward groundbreaking findings.