| Product name | ZNF420 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human ZNF420 at AA rangle: 270-350 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ZNF420; Zinc finger protein 420 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 80kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Zinc finger protein 420 encoded by ZNF420 is a KRAB-type zinc finger protein that negatively-regulates p53-mediated apoptosis. Under stress conditions, the encoded protein is phosphorylated by ATM and dissociates from p53, which activates p53 and initiates apoptosis. |
| Gene ID | 147923 |
| Alternative | ZNF420; Zinc finger protein 420 |
| Others | ZNF420 Polyclonal Antibody detects endogenous levels of ZNF420 protein. |
| Accession | Q8TAQ5 |
| Observed Band(KD) | 80 |
Fig. Western Blot analysis of K562 cells using ZNF420 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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