| Product name | ZIS Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human ZIS at AA range: 80-160 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ZRANB2; ZIS; ZNF265; Zinc finger Ran-binding domain-containing protein 2; Zinc finger protein 265; Zinc finger; splicing |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 34kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ZRANB2, also named as ZIS and ZNF265, is a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. It may interfere with constitutive 5'-splice site selection. The calculated MW of ZRANB2 is 330aa, 37 kDa or 320aa, 36 kDa. We always got the ~48 kDa in our detection. The immunogen of this antibody is 1-241aa. No other protein can be recognized by this antibody from the sequence BLAST results. |
| Gene ID | 9406 |
| Alternative | ZRANB2; ZIS; ZNF265; Zinc finger Ran-binding domain-containing protein 2; Zinc finger protein 265; Zinc finger; splicing |
| Others | ZIS Polyclonal Antibody detects endogenous levels of ZIS protein. |
| Accession | O95218 |
| Observed Band(KD) | 34 |
Fig. Western Blot analysis of various cells using ZIS Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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