| Product name | WTAP Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human WTAP at AA range: 290-370 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | WTAP; KIAA0105; Pre-mRNA-splicing regulator WTAP; Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 55kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. WTAP encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, pre-mRNA-splicing regulator WTAP is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. |
| Gene ID | 9589 |
| Alternative | WTAP; KIAA0105; Pre-mRNA-splicing regulator WTAP; Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein |
| Others | WTAP Polyclonal Antibody detects endogenous levels of WTAP protein. |
| Accession | Q15007 |
| Observed Band(KD) | 44 |
Fig. Western Blot analysis of various cells using WTAP Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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