| Product name | Wnt-1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Wnt-1 at AA range: 270-350 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | WNT1; INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 45kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. Proto-oncogene Wnt-1 have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT1 is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. |
| Gene ID | 7471 |
| Alternative | WNT1; INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog |
| Others | Wnt-1 Polyclonal Antibody detects endogenous levels of Wnt-1 protein. |
| Accession | P04628 |
| Observed Band(KD) | 45 |
Fig.1. Western Blot analysis of various cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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