| Product name | VPS35 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from VPS35 at AA range: 511-560 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Vacuolar protein sorting-associated protein 35; hVPS35; Maternal-embryonic 3; Vesicle protein sorting 35 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 92kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | VPS35 (VPS35, retromer complex component) belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. |
| Gene ID | 55737 |
| Alternative | Vacuolar protein sorting-associated protein 35; hVPS35; Maternal-embryonic 3; Vesicle protein sorting 35 |
| Others | VPS35 Polyclonal Antibody detects endogenous levels of VPS35. |
| Accession | Q96QK1 |
| Observed Band(KD) | 92 |
Fig. Western Blot analysis of Hela cells using VPS35 Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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