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Vav3 (phospho Tyr173) Polyclonal Antibody

Vav3 (phospho Tyr173) Polyclonal Antibody

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Specification

Product name Vav3 (phospho Tyr173) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Vav3 around the phosphorylation site of Y173
Host Rabbit
Reactivity Human,Mouse
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative VAV3; Guanine nucleotide exchange factor VAV3; VAV-3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 100kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background VAV3 is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Gene ID 10451
Alternative VAV3; Guanine nucleotide exchange factor VAV3; VAV-3
Others Phospho-Vav3 (Y173) Polyclonal Antibody detects endogenous levels of Vav3 protein only when phosphorylated at Y173.
Accession Q9UKW4
Observed Band(KD) 100

Image & description

Fig. Western Blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody.

Fig. Western Blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody.

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