| Product name | Troponin T-C Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Troponin T-C |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | TNNT2; Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 35kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Troponin T cardiac muscle encoded by TNNT2 is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. |
| Gene ID | 7139 |
| Alternative | TNNT2; Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT |
| Others | Troponin T-C Polyclonal Antibody detects endogenous levels of Troponin T-C protein. |
| Accession | P45379 |
| Observed Band(KD) | 35 |
Fig.1. Western Blot analysis of AD293, 4T1 cells using Troponin T-C Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse-heart, antibody was diluted at 1:100.
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