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Tropomyosin α Polyclonal Antibody

Tropomyosin α Polyclonal Antibody

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Specification

Product name Tropomyosin α Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human Tropomyosin α protein at amino acid sequence of 101-150
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 38kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TPM1 (Tropomyosin 1) is a Protein Coding gene. Diseases associated with TPM1 include Cardiomyopathy, Hypertrophic, 3 and Cardiomyopathy, Dilated, 1E. Among its related pathways are Striated Muscle Contraction and Dilated cardiomyopathy (DCM). This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in TPM1 are associated with type 3 familial hypertrophic cardiomyopathy.
Gene ID 7168
Alternative Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1
Others The antibody detects endogenous levels of Tropomyosin α
Accession P09493
Observed Band(KD) 38

Image & description

Fig.1. Western Blot analysis of 1, Mouse-lung 2, Mouse-brain 3,Mouse-spleen cells using primary antibody diluted at 1:500 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

Fig.1. Western Blot analysis of 1, Mouse-lung 2, Mouse-brain 3,Mouse-spleen cells using primary antibody diluted at 1:500 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.

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