| Product name | Transferrin Monoclonal Antibody |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB,IHC,IF, |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000;IF 1:200;IHC 1:50-300 |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Alternative | TF; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 77kD |
| Storage buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | TF encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. Transferrin may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. |
| Gene ID | 7018 |
| Alternative | TF; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin |
| Others | The antibody detects endogenous Human Transferrin protein. |
| Accession | P02787 |
Fig.1. Western blot analysis of Human serum, mAb diluted at 1:2000.
Fig.2. Immunofluorescence analysis of human lung cancer tissue. 1, Transferrin Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, Transferrin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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