| Product name | Topo IIIβ-1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Topo IIIβ-1 at AA range: 30-110 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | TOP3B; TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 96kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | TOP3B encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
| Gene ID | 8940 |
| Alternative | TOP3B; TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1 |
| Others | Topo IIIβ-1 Polyclonal Antibody detects endogenous levels of Topo IIIβ-1 protein. |
| Accession | O95985 |
| Observed Band(KD) | 96 |
Fig. Western Blot analysis of various cells using Topo IIIβ-1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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