| Product name | Topo IIIα Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Topo IIIα at AA range: 350-430 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | TOP3A; TOP3; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 115kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | TOP3A(topoisomerase(DNA) III alpha) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Gene ID | 7156 |
| Alternative | TOP3A; TOP3; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha |
| Others | Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein. |
| Accession | Q13472 |
| Observed Band(KD) | 115 |
Fig. Western blot analysis of 293T using Topo IIIα antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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