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TGFβ2 Polyclonal Antibody

TGFβ2 Polyclonal Antibody

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Specification

Product name TGFβ2 Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human TGFβ2 protein at amino acid sequence of 61-110
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:500-200;ELISA 1:10000-20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Transforming growth factor beta-2; TGF-beta-2; BSC-1 cell growth inhibitor; Cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Polyergin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 47kD,12kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TGFβ2 encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. Mutations in this gene may be associated with Loeys-Dietz syndrome. TGFβ2 encodes multiple isoforms that may undergo similar proteolytic processing.
Gene ID 7042
Alternative Transforming growth factor beta-2; TGF-beta-2; BSC-1 cell growth inhibitor; Cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Polyergin
Others The antibody detects endogenous levels of TGFβ2
Accession P61812
Observed Band(KD) 47+12

Image & description

Fig.1. Western blot analysis of Mouse-kidney 293T Mouse-lung lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.1. Western blot analysis of Mouse-kidney 293T Mouse-lung lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.

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