| Product name | TAF II p100 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human TAF II p100 at AA range: 350-430 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | TAF5; TAF2D; Transcription initiation factor TFIID subunit 5; Transcription initiation factor TFIID 100 kDa subunit; TAF(II)100; TAFII-100; TAFII100 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 87kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. Transcription initiation factor TFIID subunit 5 that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF5 encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. |
| Gene ID | 6877 |
| Alternative | TAF5; TAF2D; Transcription initiation factor TFIID subunit 5; Transcription initiation factor TFIID 100 kDa subunit; TAF(II)100; TAFII-100; TAFII100 |
| Others | TAF II p100 Polyclonal Antibody detects endogenous levels of TAF II p100 protein. |
| Accession | Q15542 |
| Observed Band(KD) | 87 |
Fig. Western Blot analysis of various cells using TAF II p100 Polyclonal Antibody.
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