| Product name | SPOP Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from SPOP at AA range: 41-90 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Speckle-type POZ protein; HIB homolog 1; Roadkill homolog 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 42kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SPOP (speckle type BTB/POZ protein) encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1. , a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of SPOP results in multiple transcript variants encoding the same protein. |
| Gene ID | 8405 |
| Alternative | Speckle-type POZ protein; HIB homolog 1; Roadkill homolog 1 |
| Others | SPOP Polyclonal Antibody detects endogenous levels of SPOP. |
| Accession | O43791 |
| Observed Band(KD) | 42 |
Fig.1. Western Blot analysis of 3T3 HEPG2 Hela cells using SPOP Polyclonal Antibody diluted at 1:1500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
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