| Product name | Splicing factor 1 (phospho Ser82) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Splicing factor 1 around the phosphorylation site of S82 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | SF1; ZFM1; ZNF162; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 68kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SF1 (splicing factor 1) encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. |
| Gene ID | 7536 |
| Alternative | SF1; ZFM1; ZNF162; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162 |
| Others | Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82. |
| Accession | Q15637 |
| Observed Band(KD) | 68 |
Fig. Western blot analysis of SH-SY5Y 293T using p-Splicing factor 1 (S82) antibody. Antibody was diluted at 1:500.
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