| Product name | SphK2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human SphK2 around the non-phosphorylation site of T614 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | SPHK2; Sphingosine kinase 2; SK 2; SPK 2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 70kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SPHK2 (sphingosine kinase 2) encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for SPHK2. |
| Gene ID | 56848 |
| Alternative | SPHK2; Sphingosine kinase 2; SK 2; SPK 2 |
| Others | SphK2 Polyclonal Antibody detects endogenous levels of SphK2 protein. |
| Accession | Q9NRA0 |
| Observed Band(KD) | 70 |
Fig.1. Western Blot analysis of 22RV1 cells using SphK2 Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of AD293 using SphK2 Polyclonal Antibody diluted at 1:500.
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